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Procyanidin—Cell Wall Interactions within Apple Matrices Decrease the Metabolization of Procyanidins by the Human Gut Microbiota and the Anti-Inflammatory Effect of the Resulting Microbial Metabolome In Vitro ArchiMer
Le Bourvellec, Carine; Bagano Vilas Boas, Priscilla; Lepercq, Pascale; Comtet-marre, Sophie; Auffret, Pauline; Ruiz, Philippe; Bott, Romain; Renard, Catherine; Dufour, Claire; Chatel, Jean-marc; Mosoni, Pascale.
B-type oligomeric procyanidins in apples constitute an important source of polyphenols in the human diet. Their role in health is not known, although it is suggested that they generate beneficial bioactive compounds upon metabolization by the gut microbiota. During apple processing, procyanidins interact with cell-wall polysaccharides and form stable complexes. These interactions need to be taken into consideration in order to better assess the biological effects of fruit constituents. Our objectives were to evaluate the impact of these interactions on the microbial metabolization of cell walls and procyanidins, and to investigate the potential anti-inflammatory activity of the resulting metabolome, in addition to analyzing the taxonomical changes which...
Tipo: Text Palavras-chave: In vitro batch fermentation; Polyphenols; Dietary fiber; 16S metabarcoding; Metabotype.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00485/59712/62805.pdf
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Production, Composition, Fatty Acids Profile and Stability of Milk and Blood Composition of Dairy Cows Fed High Polyunsaturated Fatty Acids Diets and Sticky Coffee Hull BABT
Santos,Geraldo Tadeu dos; Schogor,Ana Luiza Bachmann; Romero,Jakeline Vieira; Lima,Luciano Soares de; Matumoto-Pintro,Paula Toshimi; Grande,Paula Adriana; Kazama,Daniele Cristina da Silva; Santos,Fabio Seiji dos.
Four lactating Holstein cows were assigned to a 4 × 4 Latin square design to determine the effects of feeding sticky coffee hull (SCH) as a source of antioxidants on dairy cows fed with high PUFA diets. The treatments (on DM basis) were control diet, diet with 30 g/kg of soybean oil, diet with 30 g/kg of soybean oil and 100 g/kg of SCH, and diet with 30 g/kg of soybean oil and 150 g/kg of SCH. Inclusion of 150 g/kg of SCH decreased the crude protein digestibility. Lower values of NDF digestibility were also observed when cows were fed with 100 g/kg and 150g/kg of SCH. The digestibility of NDT was lower in the control and 150 g/kg of SCH diets. Milk production and composition did not differ among the treatments. Inclusion of SCH increased the total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: By-product; Coffea arabica; Digestibility; Flavonoids; Milk stability; Polyphenols.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400493
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Propolis from Southwest of Parana produced by selected bees: Influence of seasonality and food supplementation on antioxidant activity and phenolic profile Anais da ABC (AABC)
CALEGARI,MATHEUS A.; PRASNIEWSKI,ANACLARA; SILVA,CLEIDIANE DA; SADO,RICARDO Y.; MAIA,FABIANA M.C.; TONIAL,LARISSA M.S.; OLDONI,TATIANE L.C..
ABSTRACT Propolis produced by selected bees Apis mellifera were collected from March to June of 2013 and in March of 2015 and analyzed in order to evaluate the influence of climate, colony of origin, and food supplementation of colonies on the content of total phenolic and flavonoid by chromatographic analysis and antioxidant activity by radical scavenging of 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) methods. The Principal Component Analysis (PCA) was carried out with propolis collected in 2013 and two clusters were formed. Propolis produced in the months of March and April showed a higher content of total phenolic compounds (TPC) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Ethanolic extract; Polyphenols; Principal component analysis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100045
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Red Wine administration to Apolipoprotein E-deficient Mice reduces their Macrophage-derived Extracellular Matrix Atherogenic Properties Biol. Res.
KAPLAN,MARIELLE; AVIRAM,MICHAEL.
Proteoglycans (PGs) from the arterial extracellular matrix (ECM) contribute to the trapping of LDL and oxidized LDL (Ox-LDL) in the arterial wall, a phenomenon called "lipoprotein retention". Moreover, we have shown that subsequent to their binding to the matrix, LDL and Ox-LDL are taken up by macrophages. Oxidative stress significantly increases macrophage secretion of ECM-PGs, lipoprotein binding to the ECM and the uptake of ECM-retained lipoproteins by macrophages. The aim of the present study was to determine whether red wine administration to atherosclerotic mice would affect their peritoneal macrophage-derived extracellular matrix properties, such as the glycosaminoglycan content and the ability to bind LDL. In addition, we questioned the ability of...
Tipo: Journal article Palavras-chave: Antioxidants; Atherosclerosis; Extracellular matrix; Macrophages; Polyphenols; Proteoglycans; Red wine.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200010
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Red wine phenolic composition: the effects of summer pruning and cluster thinning Ciencia e Investigación Agraria
Cañón,Pablo M; González,Álvaro S; Alcalde,José A; Bordeu,Edmundo.
P.M. Cañon, A.S. González, J.A. Alcalde, and E. Bordeu. 2014. Red wine phenolic composition: the effects of summer pruning and cluster thinning. Cien. Inv. Agr. 41(2): 235-248. The quality of red wine is directly associated with its phenolic composition, which can be controlled using several viticultural techniques that affect the vegetative/productive balance of the plants, such as summer pruning and cluster thinning. However, these techniques may also involve high costs and production losses. This study aimed to evaluate the effects of summer pruning and cluster thinning on the phenolic compositions of grapes and wine over three consecutive seasons. The treatments included long (120 cm shoot length) and short (60 cm shoot length) summer pruning and 50%...
Tipo: Journal article Palavras-chave: Cluster thinning; Polyphenols; Summer pruning; Vegetative/productive balance; Vitis vinifera L..
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200010
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Scale-up in the synthesis of nanoparticles for encapsulation of agroindustrial active principles Ciência e Agrotecnologia
Duarte,Dirliane Santos; Nascimento,José Augusto de Almeida; Britto,Douglas de.
ABSTRACT Given the potentiality of nanoparticles (NP) to load substances as active principle of drugs, cosmetics and food, there is interest in increasing its production. Particularly in the agroindustrial area, the byproducts are source of functional compounds that must be stabilized by encapsulation, for example, to allow its application. Considering the amount of generated byproducts, it is necessary to understand the encapsulation synthesis in a high scale dimension. In this way, the active compounds vitamin C (from the byproduct of the acerola processing) and polyphenols (from the byproduct of grape processing) were nanoencapsulated into chitosan NP at three reactional volumes: 0.1; 1.0 and 10.0 dm3. The NP were characterized for yield, particle size,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitamin C; Polyphenols; Byproduct; Chitosan.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100406
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Simple and robust DNA extraction method for the large-scale analysis of genotypes containing high polyphenolic content, such as landracesof Solanum tuberosum and Zea mays Ciencia e Investigación Agraria
Barra,Macarena; Salazar,Erika; Beltrán,María; Sagredo,Boris.
The extraction of high-quality DNA is essential for studies conducted at the molecular level in species with an abundance of contaminants in their tissues, such as some landraces of potato and maize, in which it is difficult to extract good-quality genomic DNA. Compounds such as polyphenols interfere with the amplification of DNA during polymerase chain reaction (PCR), which makes it difficult to conduct PCR-based studies ofmolecular markers. This article describes a simple and robust protocol for DNA isolation that was applied to potato and maize landraces and resulted in a high DNA concentration with excellent purity and quality. This method is based on the method of Krizman et al. (2006) with some modifications and was tested on potato leaves and young...
Tipo: Journal article Palavras-chave: Activated charcoal; DNA extraction; Genomic DNA; Polyphenols.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300019
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Species-Specific Antioxidant Power and Bioactive Properties of the Extracts Obtained from Wild Mediterranean Calendula Spp. (Asteraceae) ArchiMer
Messina, Concetta Maria; Troia, Angelo; Arena, Rosaria; Manuguerra, Simona; Ioannou, Theodora; Curcuraci, Eleonora; Renda, Giuseppe; Hellio, Claire; Santulli, Andrea.
n this study we focused on four taxa of the genus Calendula (C. maritima, C. suffruticosa subsp. fulgida, C. arvensis, and the hybrid between the first two ones), collected in Mediterranean area (Sicily). Six extracts for each species were obtained using solvents with increasing polarity (hexane, ethanol 80%, acetone 70%, and water) and through extraction by supercritical fluids (SFE). It has been observed that the solvent with the highest extraction efficiency was ethanol 80% for all species. However, SFE extracts showed high antioxidant activity comparable to the ethanol 80% extract (polyphenol, DPPH, and reducing power method). These findings were confirmed by in vitro analysis (MTT assay) where it was observed that the tested concentration (24 µg/mL),...
Tipo: Text Palavras-chave: Calendula species; Marigold; Supercritical fluid extraction; Bioactivity; Antioxidants; Polyphenols.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00591/70336/68389.pdf
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Standardization of the quality control parameters of the Tamarindus indica L. soft extract Plantas Medicinales
Rodríguez-Amado,Jesús; Pérez Rosés,Renato; Escalona-Arranz,Julio César; Lafourcade Prada,Ariadna; Sierra González,Gustavo.
Introduction: Tamarindus indica L or Tamarind as commonly known is a medicinal plant witha broad spectrum of application in medicine. Tamarind is the most useful plant for liver disorders treatment in Cuba and The Caribbean. People use it as decoction and have to take up to four liters a day of this preparation, because the use of alcohol in the Tamarind fluid extracts prevent its use in patient with lever desorders. Objective: the aim of this study was the preliminary establisment of quality control parameters of the Tamarindus indica L. soft extract as a novel alcohol-free active ingredient for solids pharmaceutical formulation. Methods: organoleptics properties, total solids, relative density, pH, and total ashes were stablished. The polyphenols content...
Tipo: Journal article Palavras-chave: Soft extract; Polyphenols; Flavonoids; Antioxidant; Tamarind; Tamarindus indica.
Ano: 2012 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962012000100012
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The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance Ciencia e Investigación Agraria
Curifuta,Macarena; Vidal,Jorge; Sánchez-Venegas,Jaime; Contreras,Aliro; Salazar,Luis A; Alvear,Marysol.
Propolis has been used for millennia for its antimicrobial and pharmaceutical properties, whereas its use as an agricultural antifungal agent has only recently been assessed. The chemical characteristics of six ethanolic extracts of Chilean propolis (EEP-1 to EEP-6) were evaluated. The total polyphenols ranged between 7.8 and 42.3 mg mL-1 equivalents of a 2:1 pinocembrin: galangin standard, and the total flavonoid contents ranged between 4.0 and 19.7 mg mL-1 equivalents of quercetin. A high-performance liquid chromatographic (HPLC) analysis allowed the identification of caffeic acid, myricetin, quercetin, kaempferol, apigenin, pinocembrin, galangin, caffeic acid phenethyl ester (CAPE) and rutin. The EEP-3 extract was selected for additional studies based...
Tipo: Journal article Palavras-chave: Alternaria alternata; Antifungal activity; Botrytis cinerea; Flavonoids; Fusarium sp.; Penicillium expansum; Polyphenols; Propolis.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200011
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Total phenolics, flavonoids, tannins and antioxidant activity of lima beans conserved in a Brazilian Genebank Ciência Rural
Agostini-Costa,Tânia da Silveira; Teodoro,Ana Flávia Pádua; Alves,Rosa de Belem das Neves; Braga,Leandro Ribeiro; Ribeiro,Ieler Ferreira; Silva,Joseane Padilha; Quintana,Luciane Gomes; Burle,Marília Lobo.
The objective of this study was to characterize for the first time polyphenols and DPPH (2-diphenyl-1-picryhydrazyl radical) antioxidant activity in commonly cultivated accessions of Phaseolus lunatus from an ex situ germplasm collection maintained by Embrapa, in Brazil. Furthermore, the study aimed to detect changes in total polyphenols, total flavonoids and condensed tannin for the same accessions after regeneration in a greenhouse. The results showed the diversity of the lima bean collection for phenolic compounds, which were strongly correlated with antioxidant activity. Lima beans accessions with the highest polyphenols and antioxidant activity were those with colored seeds. Conservation through cold storage of P. lunatus seeds in a cold chamber in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus lunatus; Genetic resources; Germplasm collection; Leguminosae; Polyphenols.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000200335
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